Effect of GNF-7 suppression of AKT and/or GCK on induction of apoptosis and cell cycle. (A) Indicated cell lines were treated with GNF-7 for 24 hours and percentage of annexin V-positive cells are shown. Data are represented as mean ± standard error of the mean (SEM). (B) Indicated cell lines were treated with 0.5 μM of GNF-7 (0.1 μM for MOLT3) for 24 hours and expression of proteins involved in apoptosis are shown. (C) Expression of BCL2 in MOLT3 cells and MOLT3-BCL2 cells 24 hours after treatment with DMSO or MK-2206 (0.2 μM) or NG25 (0.03 μM), or a combination of MK-2206 (0.2 μM) and NG25 (0.03 μM) or GNF-7 (0.1 μM). (D) Percentage of annexin V-positive cells after 24 hours in the same experiment (as shown in C). (E) Proliferation of MOLT3 cells and MOLT3-BCL2 cells with presence of MK-2206 and NG25. (F) Proliferation of MOLT3 cells and MOLT3-BCL2 cells characterized by KD of GCK by shRNA with presence of MK-2206. Data are represented as mean ± SEM. GFP, green fluorescent protein; mut, mutant; wt, wild type. *P < .05; **P < .01