Comparing the function and targets of GNF-7 in additional leukemia cell lines. (A) Proliferation of WT and mutant NRAS-expressing AML or ALL (acute lymphoblastic leukemia) cell lines treated with MK-2206 and NG25. Data are represented as mean ± standard error of the mean (SEM). (B) Proliferation of AML or ALL cell lines treated with MK-2206 and characterized by KD of GCK by shRNA. Data are represented as mean ± SEM. (C) Validation of the KD efficiency of GCK by shRNAs used (as shown in B). (D) Proliferation of THP-1 cells and THP1-GCK-WT cells treated with MK-2206 and characterized by KD of GCK by shRNA. Data are represented as mean ± SEM. (E) Validation of the KD efficiency of GCK by shRNA (used in D) in THP-1 cells and THP1-GCK-WT cells. GFP, green fluorescent protein; mut, mutant; n.s., nonsignificant; wt, wild type. *P < .05.