Figure 3
Deletion of Rac genes in perivascular space leads to abnormal trabecular bone formation in the medullary cavity. Femurs from TG WT and TG Rac1Δ/ΔRac3−/− TG Rac1Δ/ΔRac3−/− mice were prepared and quantification performed as detailed in Material and methods. (A) Hematoxylin and eosin staining of femurs of TG WT and TG Rac1Δ/ΔRac3−/− mice. The scale bar represents 500 μm. Photomicrographs were viewed at 40× original magnification using a Nikon Eclipse 80i microscope, and analyzed with NIS Elements Version 2BR imaging software. (B) Quantification of trabecular bone numbers and (C) trabecular bone volume in medullary cavity space. Data generated using NIS Elements AR software. Data represent mean ± SD, N = 3 animals per group, **P < .01, ***P < .001. (D) Micro-CT images of distal and proximal femoral trabecular bone structure in TG WT and TG Rac1 Δ/ΔRac3−/− mice.

Deletion of Rac genes in perivascular space leads to abnormal trabecular bone formation in the medullary cavity. Femurs from TG WT and TG Rac1Δ/ΔRac3−/− TG Rac1Δ/ΔRac3−/− mice were prepared and quantification performed as detailed in Material and methods. (A) Hematoxylin and eosin staining of femurs of TG WT and TG Rac1Δ/ΔRac3−/− mice. The scale bar represents 500 μm. Photomicrographs were viewed at 40× original magnification using a Nikon Eclipse 80i microscope, and analyzed with NIS Elements Version 2BR imaging software. (B) Quantification of trabecular bone numbers and (C) trabecular bone volume in medullary cavity space. Data generated using NIS Elements AR software. Data represent mean ± SD, N = 3 animals per group, **P < .01, ***P < .001. (D) Micro-CT images of distal and proximal femoral trabecular bone structure in TG WT and TG Rac1 Δ/ΔRac3−/− mice.

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