Aging gene-expression profiles in CD4⁺ T cells from patients with ICL. (A) Regulated genes across the samples were hierarchically clustered according to their mean-normalized intensity across all samples. Blue and pink samples correspond to CD4⁺ T cells from healthy subjects (n = 8) and patients with ICL (n = 9), respectively. (B) Venn diagram of analyzed, expressed, and regulated genes in CD4⁺ T cells for patients with ICL vs healthy subjects. Upregulated and downregulated genes are indicated by red and green colors, respectively. (C) Results extracted from the Gene Ontology analysis, KEGG pathway, and list of genes regulated in healthy T cells during aging²⁸  were combined to delineate a specific T-cell aging pathway in ICL. ¹Genes that are significantly regulated in another hierarchical clustering. (D) Ten representative genes of the upregulated group and 6 of the downregulated group were monitored by quantitative PCR in healthy (n = 12), SARC (n = 8), and ICL (n = 12) CD4⁺ T cells. Each individual sample was run in triplicate. Results are expressed as mRNA levels after normalization to GAPDH mRNA levels. *P < .05, **P < .005, and ***P < .0005 compared with healthy CD4⁺ T cells (as determined using the Mann-Whitney U test).