Mobility of CD95 within the plasma membrane is regulated by palmitoylation. FRAP assays were performed to investigate the impact of palmitoylation on the receptor’s mobility on the plasma membrane. A typical experiment is shown for CD95-eYFP and CD95-eYFP mut (A). Comparison of wild-type (palmitoylated) CD95 with mutated (C199S, unpalmitoylated) CD95 showed that the mobile fraction is significantly higher in cells expressing the unpalmitoylated receptor (n = 15), while t1/2 is unaffected (B-C). Means are given with their SEM. The statistical significance was determined using the Student t test. *P < .05, **P < .01, or ***P < .001 were considered significant.