CD95 and APTs interact at the plasma membrane. FLIM-FRET experiments of CD95 with APT1 and APT2 demonstrate that CD95 directly interacts with both proteins (A). The interaction is dependent on the palmitoylation of cysteine 199 (B). Lifetime of the donor (CD95-eYFP) at the plasma membrane and in the cytoplasm is significantly reduced after incubation with the acceptor (APT1- or APT2-mCherry) (C). Means are given with their SEM. The statistical significance was determined using the Student t test. *P < .05, **P < .01, or ***P < .001 were considered significant.