Extent and durability of fucosylation of CB cells. (A) Mean fluorescence intensity of fucosylated (white) and untreated (gray) CB cells. The degree of fucosylation (measured with the HECA antibody) on CB-CD34⁺ cells, megakaryocytes (CD61⁺CD45⁺), NK cells (CD3⁻CD56⁺), and monocytes (CD14⁺) was increased compared with that of the corresponding untreated cells (gray), whereas neither T cells (CD3⁺) nor B cells (CD19⁺) showed increased fucosylation. (B) Stable HECA expression (fucosylation) on (a) CD34⁺ cells, (b) megakaryocytes (CD45⁺CD61⁺), and (c) NK cells (CD3⁻CD56⁺) for up to 24 hours after the fucosylation procedure.