β2GPI is complexed with HLA-DR in endothelial cells of vessels in uterine decidual tissues obtained from APS patients. (A-C, E-G, I-K, M-O) β2GPI and HLA-DR are coexpressed in endothelial cells of vessels in uterine decidual tissues of APS patients, but not patients without APS. Tissue sections of (A-D) uterine decidua, (E-H) chorionic villi, and (I-L) villous core from APS patients and (M-P) decidua from APS-free patients were costained with anti-HLA-DR Ab (red, A, E, I, M) and anti-β2GPI Ab (green, B, F J, N). The images were merged to show colocalization of β2GPI and HLA-DR (C, G, K, O). PLA signals (red) between HLA-DR and β2GPI were analyzed in tissues of APS patients and those without APS (D, H, L, P). Scale bars, 50 μm. Data are representative of 3 independent experiments.