Coagulopathy induced by infusion of BDMPs into uninjured mice. (A) Blood samples were collected from mice infused with BDMPs or PBS and tested for PS-dependent clot formation (n = 12, 1-way ANOVA, **P = .036, *P < .001 compared with PBS). (B) Levels of fibrinogen were measured in the plasma samples collected for studies in A (n = 12, 1-way ANOVA, *P < .001 compared with PBS). Sections of formaldehyde-fixed lungs, kidney, and heart from mice injected with (C-E) BDMPs or (F-H) PBS were stained with phosphotungstic acid hematoxylin (dark blue; the dye also stains muscle cells). The left panel shows fibrin deposition in the microvasculature of the (C) lung, (D) kidney, and (E) stromatic vessels of the heart from mice injected with BDMPs (arrows). Arrowheads indicate intact walls of fibrin deposited vessels (bar = 25 μm). (F-H) Fibrin deposition was not detected in organs collected from mice receiving PBS (bar = 25 μm). Images are representative of 6 mice injected with BDMPs and 5 with PBS.