BCT-100 halts proliferation and cell cycle arrest. (A) BCT-100 halts AML cell division. CFSE-labeled cell lines were cultured in the presence of 600 ng/mL BCT-100. Representative histogram plots shown. Independent experiments were performed on 2 separate occasions. (B) Cell lines were cultured with BCT-100 (0-2000 ng/mL) for 72 hours. AML proliferation was measured by 3H-thymidine incorporation after 72 hours. Data are representative of 2 independent experiments. BCT-100 causes a dose-dependent decrease in AML proliferation. (C) AML cell lines were cultured with 600 ng/mL BCT-100. Cell cycle analysis was performed after 72 hours. BCT-100 increases the percentage of cells in G0/G1 arrest. Representative histogram plots for untreated and treated HL-60 shown. Independent experiments were performed on 4 separate occasions. (D) Table showing the relative percentages of cells in G0/G1, S, G2/M based on flow cytometry cell cycle analysis.
