LMP2A downregulates the expression of CIITA, E47, and PU.1. (A) Akata and BJAB cells were infected with LMP2A-expressing lentiviruses. Expression of CIITA and LMP2A was detected by western blotting. (B) The expression of the CIITA-PI, CIITA-PIII, CIITA-PIV, and LMP2A transcripts was measured by RT-PCR analysis. Raji cells were used as a positive control for the CIITA promoters. (C) Expression of E47, PU.1, IRF4, IRF8, and LMP2A was measured by RT-PCR analysis. (D) Expression of E47, PU.1, and LMP2A was detected by western blotting. (E) Akata and BJAB cells were infected with Zta-expressing lentiviruses. Expression of E47, PU.1, and Zta was detected by western blotting. β-actin was detected as an internal control. Each experiment was performed 3 times, and 1 representative is shown.