Gene expression analysis in peripheral blood, bone marrow, and spleen of transgenic mice and controls. Quantitative PCR measuring the fold changes in mRNA expression of selected megakaryopoietic differentiation markers (A: Gata1, NF-E2, and Gp1bα) and Stat1 target genes (B: Usp18, Ifi27l2α, and Stat1) are shown and normalized against GusB mRNA. One-way ANOVA was used for comparisons with subsequent Bonferroni post-test (*P < .05).