Phospho-flow analysis of signaling in bone marrow and spleen cells. Mice were euthanized 25 weeks after starting tamoxifen. Intracellular detection by flow cytometry of phospho(Y701)Stat1 and phospho(Y705)Stat3 in total and in CD41⁺ (A) bone marrow and (B) spleen cells stimulated or not with IL-6 or Tpo. Fold increase (unstimulated WT control set to 1) of positive phosphorylated cells was calculated (n = 3 per group). One-way ANOVA was used for comparisons with subsequent Bonferroni post-test (*P < .05).