Flow cytometry analysis of hematopoietic stem cells and early megakaryopoietic progenitors. Mice were euthanized 25 weeks after starting tamoxifen. (A) Analysis of bone marrow cells with flow cytometry scattergrams showing the gating strategy in lineage depleted samples (left). LSK, lineage⁻Sca1⁺ckit⁺ cells; LK, lineage⁻Sca1⁻ckit⁺; megakaryocytic progenitors (MkPs), lineage⁻Sca1⁻ckit⁺/CD150⁺CD41⁺. Histograms of the means of hematopoietic progenitors from bone marrow ± SEM (n = 2 mice per genotype) (right). (B) Analysis of spleen cells, annotation as in A. One-way ANOVA with subsequent Bonferroni post-test was used, and *P < .05 is indicated.