American Society of Hematology

Figure 6

$Figure 6. In vitro expansion of human stress erythroid progenitors. (A) Human unfractionated bone marrow cells were cultured in SEDM (indicated by C) or SEDM lacking the indicated factors for 5 days and plated in methylcellulose media containing Epo alone. Stress BFU-E colonies were scored after 12 days. Data are representative of 3 independent experiments using bone marrow from 3 different human donors. Below, representative pictures of BFU-E colonies are shown. Photos were taken with a Nikon ECLIPSE TE200 microscope at 4× magnification with the camera software DP2-BSW. (B) Flow cytometry analysis of untreated human bone marrow (left), after culture for 7 days in SEEM (middle), and after the cells were switched in SEDM (right). The cells were gated on KIT+, and the expression of CD34 and CD133 was analyzed.$

In vitro expansion of human stress erythroid progenitors. (A) Human unfractionated bone marrow cells were cultured in SEDM (indicated by C) or SEDM lacking the indicated factors for 5 days and plated in methylcellulose media containing Epo alone. Stress BFU-E colonies were scored after 12 days. Data are representative of 3 independent experiments using bone marrow from 3 different human donors. Below, representative pictures of BFU-E colonies are shown. Photos were taken with a Nikon ECLIPSE TE200 microscope at 4× magnification with the camera software DP2-BSW. (B) Flow cytometry analysis of untreated human bone marrow (left), after culture for 7 days in SEEM (middle), and after the cells were switched in SEDM (right). The cells were gated on KIT⁺, and the expression of CD34 and CD133 was analyzed.

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