![Figure 1. Cell-binding assays evaluating the absolute (counts per minute [cpm]) binding of 1F5-B10 labeled with either 211At or 125I with or without unlabeled blocking antibodies using Ramos and Jurkat cells confirm the antigen-specific cell binding of radiolabeled 1F5-B10 conjugates. (A) [211At]1F5-B10 binding to Ramos cells was blocked by preincubation with unlabeled anti-CD20 antibodies (2321.0 ± 475.1 vs 409.0 ± 27.5 CPM; 82% blocking). (B) Similar blocking was also observed when the 1F5-B10 was labeled with 125I (14 642 ± 3621 vs 679 ± 60.6 CPM; 95% blocking). No difference was seen between the binding of 1F5-B10 antibody to the CD20-negative Jurkat cells in the presence or absence of blocking anti-CD20 antibodies. PBS, phosphate-buffered saline.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/125/13/10.1182_blood-2014-11-612770/4/2111f1.jpeg?Expires=1722792571&Signature=ZbvukpC6xCOStQoKoleB3YaszkI5-imJoZqE-~lyFpci4BuymKu-AWQ~bugprz2Az~jnnV9QDl~dy7ORD2ppgBxUOcPXMAo30OJfuVyKFKOb~557N7qmHZVuYsesg99a2Z~e6NFrwaA8s3Sz910SOqhXsHruazpAf91De0W6LL4iDJYtufoEqV4ZIQhMTW4FB8tXegtNMeuPYOTQiy-sqzIedcTKSSyZAnHRGz1eBAAADW4m8RfPaqSXhv6ueKF~6CK5xf92EFvsOuWAuNVCyOmm4PBM2vf1ZPogRyw3EOh5nAG-PpFlZY-Vid~7582N60OTmF~joba1g-UMLpf4~w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell-binding assays evaluating the absolute (counts per minute [cpm]) binding of 1F5-B10 labeled with either 211At or 125I with or without unlabeled blocking antibodies using Ramos and Jurkat cells confirm the antigen-specific cell binding of radiolabeled 1F5-B10 conjugates. (A) [211At]1F5-B10 binding to Ramos cells was blocked by preincubation with unlabeled anti-CD20 antibodies (2321.0 ± 475.1 vs 409.0 ± 27.5 CPM; 82% blocking). (B) Similar blocking was also observed when the 1F5-B10 was labeled with 125I (14 642 ± 3621 vs 679 ± 60.6 CPM; 95% blocking). No difference was seen between the binding of 1F5-B10 antibody to the CD20-negative Jurkat cells in the presence or absence of blocking anti-CD20 antibodies. PBS, phosphate-buffered saline.
