The effects of VT on Gal3BP and gal3 in vein wall and thrombus (local). Gene expression and Histology. (A) Analysis of gal3BP and gal3 in vein wall by qRT-PCR: gal3BP and gal3 mRNA isolated from the IVC (vein wall) of non-VT (n = 8) or 48 hours VT (n = 12) WT mice were quantified using qRT-PCR. Gene expression was quantified relative to β-actin. (B) H&E and IHC for gal3. H&E stain and IHC for gal3 in both non-VT and VT conditions. Arrows indicate positive stained cells. Of note, the number of gal3 positive cells were increased on VT condition.