The effects of VT on Gal3BP and gal3 in blood-circulating elements (systemic) or thrombus and vein wall (Local). Western blot and quantification: western blot analysis of gal3BP and gal3 in RBCs (n = 1 per band), WBCs (n = 5 per band), PLTs (n = 3 per band), procoagulant microparticles (n = 3 per band), thrombus (n = 1 per band), and vein wall (n = 1 per band) collected from non-VT (–) (n = 21) or VT (+) (n = 25) mice 48 hours postthrombosis. Actin was used as a loading control. Quantification of blot intensity relative to loading control is shown below. gal3BP, galectin 3–binding protein; gal3, galectin 3; gal3m, gal3 monomer; gal3MD, gal3 multimer dimer; gal3MT, gal3 multimer trimer; gal3d, gal3 degradation product; IVC, vein wall; MP, procoagulant microparticles.