The role of C/EBPε acetylation for C/EBPε transcriptional activity during neutrophil differentiation. Regulation of C/EBPε acetylation, most importantly lysines 121 and 198, by the lysine acetyltransferase p300 and the lysine deacetylase SIRT1 is involved in the regulation of DNA binding and determines C/EBPε transcriptional activity and the subsequent expression of C/EBPε target genes during neutrophil development. In the absence of C/EBPε acetylation, represented by the lysine dead mutant, C/EBPε activity is abrogated, resulting in a block in neutrophil differentiation.