Anti-ERp5 antibody inhibits thrombus formation in vivo. (A) Anti-CD42b antibody labeled with DyLight 649 (0.1 µg/g body weight) and anti-fibrin-specific antibody labeled with Alexa Fluor 488 (0.5 µg/g body weight) were infused into a mouse 5 to 10 minutes prior to arteriolar injury. Preimmune IgG at 3 µg/g body weight (left) or anti-ERp5 antibody at 1 µg/g body weight (middle) and 3 µg/g body weight (right) were infused intravenously 20 minutes prior to injury. Representative binarized images of the fluorescent signal from platelets (red) and fibrin (green) over 180 seconds after laser-induced vessel wall injury show inhibition of thrombus formation with increasing concentrations of anti-ERp5 antibody. Median integrated platelet (B) and fibrin (C) fluorescence over time is shown before (1) vs after infusion of control IgG, 3 µg/g body weight (2); after anti-ERp5 antibody, 1 µg/g body weight (3); and after anti-ERp5 antibody, 3 µg/g body weight (4). **P < .01.