Increases of tolerogenic DCs in hemophilia B mice. (A) Flow cytometry analysis of CD11c and PDCA-1 staining in the spleen, MLNs, and PPs in hemophilia B mice following oral delivery of FIX plant material and IV challenge with FIX. Control groups were fed with WT plant material (wt fed) or with FIX material but not challenged with IV injections of FIX (FIX fed no injection). (B) CD103 and CD11c staining of MLN and PP cells of the same mice. Data points are for individual mice. Averages ± SEM are also indicated (n = 3-5 per experimental group). Shown are data from 2 independent experiments that showed similar results.