NKC gene expression in NKR-P1B-deficient mice. (A) Splenic NK cells (DX5⁺TCRβ^–) from WT, NKR-P1B-deficient, and heterozygous littermate mice were analyzed for expression of the indicated NK cell receptors encoded in the NKC. Representative plots from 1 of 6 to 7 mice are shown. The percentage of positively stained NK cells is indicated. (B) Downmodulation of NKG2D on splenic NK cells (NK1.1⁺TCRβ^–) but not NKT cells (NK1.1⁺TCRβ⁺) from NKR-P1B-deficient mice. (C) Graphical representation of relative median fluorescence intensity (MFI) of NKG2D on NK cells from WT and NKR-P1B-deficient littermate mice (n = 6 mice). Statistical analysis was performed by Student t test, and the P value is indicated. (D) NKG2D expression on NKR-P1B⁺ and NKR-P1B⁻ subsets of NK cells from the spleen of a WT B6 mouse.