PAD4^−/− mice are equally as susceptible to polymicrobial sepsis as their PAD4^+/+ littermates in the CLP model. Mice were subjected to low-grade CLP or sham operation (Sham). (A) Survival curves indicating similar survival rates of PAD4^−/− mice and their PAD4^+/+ littermates in low-grade CLP. (B) Loss of body temperature was similar in PAD4^−/− and PAD4^+/+ mice. (C) Weight loss was comparable in PAD4^+/+ vs PAD4^−/− mice, although sham-operated mice lost minimal body weight. (D) Bacterial load was measured in peritoneal lavage fluid, blood, and liver and lung homogenates. Minimal CFUs were detected in control nonoperated mice. Genotypes and treatments were as indicated. No statistical differences between PAD4^+/+ and PAD4^−/− mice were detected. NS, not significant. PAD4^+/+, black; PAD4^−/−, red.