Gene networks regulated by Utx in T-ALL. (A) Differentially expressed genes (FC >1.5; P < .05) between Utx knockdown and control murine leukemias are represented in a heat map. A selection of genes is shown in rows and each column represents 1 individual mouse leukemia sample. The scale bar shows color-coded differential expression from the mean in standard deviation units with red indicating higher levels and blue lower levels of expression. (B) Unbiased GSEA of gene expression signatures associated with murine leukemias driven by loss of Utx or NOTCH1 only vector controls. Gene sets involving early T-lymphocytes (P < .01) are significantly enriched in Utx-driven leukemias. (C) H3K27me3 ChIP-seq profiles at 2 specific gene loci (Lzts2 and Pcgf2) in murine leukemias driven by loss of Utx or NOTCH1 only vector controls. (D) GSEA of transcripts significantly downregulated upon Utx knockdown in murine leukemias (FC >1.5, P < .05; 252 probes) in gene expression signatures obtained from MOHITO samples driven by loss of Utx. Heatmap displays the TOP 25 leading edge of this gene set in Utx-driven MOHITO samples. FDR, false discovery rate; NES, negative enrichment score.