nBMT enhances cortical and trabecular bone architecture in MPS I mice. Micro-CT evaluation of femurs from 37-week-old male WT (n = 3), MPS I (n = 3), and MPS I nBMT-hi (n = 5) mice. (A) Representative 2D and 3D reconstructed images of trabecular bone and of midshaft cortical bone (transaxial section). (B) Bone mineral volume (BV/TV), trabecular separation (Tb. Sp.), trabecular number (Tb. N.), and cortical bone thickness (Cort. Thick.) were measured using Skyscan CT-analyzer software and the data presented as dot plot. *P < .05 with Wilcoxon nonparametric unpaired test, 1 side. (C) Representative pictures of the femur cortical bones of MPS I and MPS I nBMT-hi mice (hematoxylin and eosin staining), as indicated. The inner surface of the cortex is on the left. Magnifications, ×20 and ×40. (D) Representative pictures of the proximal tibia and distal femoral growth plate of MPS I, WT nBMT, and MPS I nBMT-hi mice (Toluidine blue stain, ×40).