nBMT prevents bone defects in MPS I mice. (A) Skull morphology (on the left) and radiographs (on the right) of 37-week-old WT, MPS I, WT nBMT, and MPS I nBMT-hi mice. Signs of skull abnormalities include blunted snouts with a loss of the fine taper to the face. (B) Measurements of maximum skull width and zygomatic arch width. All measures were performed on radiographic images of WT (n = 8; 4 males and 4 females), MPS I (n = 8; 4 males and 4 females), WT nBMT (n = 8; 5 males and 3 females), and MPS I nBMT-hi mice (n = 7; 4 males and 3 females), as shown on the right side of each chart. The median values relative to WT mice and minimum/maximum values are shown. *P < .05; **P < .01; ***P < .001 with Wilcoxon nonparametric unpaired test, 2 sides. (C) Representative examples of femur radiographs of WT, MPS I, WT nBMT, and MPS I nBMT-hi mice. (D) Measurements of femur, humerus, tibia, and radius-ulna thicknesses. The pictures on the right side of each graph indicate how measures were calculated. The median values relative to WT mice and minimum/maximum values are shown. *P < .05; **P < .01; ***P < .001 with Wilcoxon nonparametric unpaired test, 2 sides.