Redirection of TH17 cells with SS1-CARs. (A) Schematic representation of a panel of chimeric receptors that contain the SS1 single chain fragment and differ in the transmembrane and the intracellular domains. (B) Schematic of the experimental protocol. Peripheral blood CD4+ T cells were stimulated with antibodies to CD3/ICOS beads and cultured under TH17 polarizing conditions. Human IL-2 was added 3 days after activation. T cells were transduced with lentiviral vectors 24 hours following stimulation. After T cells rested down, they were cryopreserved. For functional assays, T cells were thawed and stimulated with surrogate antigen in media without cytokine supplementation. (C) Surface expression of the SS1 scFv fusion proteins on human CD4+ T cells at the time of functional evaluation.
