Figure 7
Figure 7. eNAMPT activities are independent of the enzymatic activities. (A-F) NLCs were treated (30 minutes) in different conditions and then stained for the presence of p-STAT3 (green fluorescence). DAPI (blue) was used to visualize nuclei. (G) Cumulative data (n = 5) are shown. (H) Box plots representing the quantification of CD11b+ NLCs count (n = 10) and the corresponding fluorescence pixel intensity (I) in the indicated conditions. (J) CD11b staining in NLC preparation by immunocytochemistry (upper panels, original magnification ×20) and by immunofluorescence (green, bottom panels, original magnification ×63).

eNAMPT activities are independent of the enzymatic activities. (A-F) NLCs were treated (30 minutes) in different conditions and then stained for the presence of p-STAT3 (green fluorescence). DAPI (blue) was used to visualize nuclei. (G) Cumulative data (n = 5) are shown. (H) Box plots representing the quantification of CD11b+ NLCs count (n = 10) and the corresponding fluorescence pixel intensity (I) in the indicated conditions. (J) CD11b staining in NLC preparation by immunocytochemistry (upper panels, original magnification ×20) and by immunofluorescence (green, bottom panels, original magnification ×63).

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