Megakaryocyte differentiation, location and ploidy status are not affected in dKO mice. (A-B) Percentage of megakaryocytes at consecutive stages of differentiation (I-V, as detailed in supplemental Figure 1) of nucleated bone marrow and spleen cells, and total bone marrow and spleen cell counts. (C-D) Ploidy staining of CD41-positive bone marrow and spleen megakaryocytes (as detailed in supplemental Figure 2). (E) Representative images of bone marrow cryosections, immunostained with CD41-PE (megakaryocytes), CD31-FITC (megakaryocytes and endothelial cells), and Phalloidin-CF633 (actin) to visualize their location and proximity to blood vessels in the bone marrow. Low and high magnifications are shown.