WGS identifies JAK1 mutations in 2 out of 4 index T-PLL cases. (A-B) The total individual reads supporting variant calling of the JAK1 p.V658F (A) and p.S703I (B) mutations in index T-PLL samples are shown. Nucleotides with a deviation from the reference sequence are highlighted. The mutations as well as a synonymous single-nucleotide polymorphism (A) are boxed. Dots in individual reads (A-B) represent unsequenced nucleotides (as opposed to sequence gaps) and are intrinsic to the self-assembling DNA nanoarray next-generation sequencing platform and unchained base-reads analysis approach used for WGS (see supplemental Methods for further details). (C-D) Sanger resequencing confirmation of the somatic acquisition of these mutations is shown.