Mutations in EVI-r AMLs. (A) Clinical, morphologic, cytogenetic, and mutational data in EVI1-r AML samples. LOH, loss of heterozygosity. (B) Summary of commonly mutated pathways. (C) IKZF1 protein and mutations. Four N-terminal (DNA-binding domain) and 2 C-terminal (dimerization domain) zinc fingers are represented by blue cylinders. (D) Distribution of IKZF1 isoforms in EVI1-r samples. The only sample expressing isoform NM_001220772 (Ik6) harbors compound heterozygous mutations of IKZF1. (E) IKZF1 expression levels in EVI1-r AMLs with and without monosomy 7. Blue dots represent samples with IKZF1 N159S mutation; orange dot indicates IKZF1 frameshift mutation. Analysis is performed with all 12 specimens except for D, where n = 9.