Expression of full-length TIF-IA protein (TIF-FL) and TIF-90 in normal, leukemic, and proliferating hematopoietic cells. (A) Expression of TIF-FL and the TIF-90 variant in cancer cell lines and primary leukemic cells (AML, n = 10) (top) and in representative individual normal bone marrow and leukemic cells (bottom) by western blot. (B) Quantitative expression of (1) TIF-FL mRNA, (2) TIF-90 mRNA, (3) pre-rRNA synthesis, and (4) Pol I occupancy of the rDNA promoter by qPCR in bone marrow mononuclear cells from normal individuals and from patients with AML. The bar indicates the average value, and the ends of the whiskers represent minimum and maximum values. Significance was determined using the Student t test. (C) Expression of TIF-FL and TIF-90 transcripts and protein in normal peripheral blood mononuclear cells from 2 individuals before and 22 hours after stimulation with phorbol ester and ionomycin: RT-PCR (left); western blot (right).