Antisense transcripts in platelets. Shown are positive and negative strand coverage graphs of the 5′ end of CD109 and of AK124950, an antisense transcript in platelets. Positive strand reads are on the top panel in blue, and negative strand reads are in the bottom panel in red. Both the expression of CD109 and the expression of an antisense transcript (AK124950, surrounded by the red box) transcribed from the opposite strand of DNA at the 5′ end of CD109 can be inferred when the read orientation is taken into account. See it yourself: To visualize CD109 and its antisense transcript in platelets via the UCSC Genome Browser, follow the Web link > access instructions for site 3 or 4 (see Table 1). Search for CD109 or AK124950 in the browser. Note that only stranded data (only site 3 or 4 of Table 1) can distinguish antisense from sense transcripts.