Low CD8⁺ T_scm in patients after allo-SCT. CD8⁺ T cells of (○) healthy donors (HD; n = 6) and (●) allo-SCT patients (n = 8 to 9) were analyzed for their differentiation status by flow cytometry. (A) Representative plot showing subset discrimination. (B) Summary of memory subsets within CD8⁺ T cells. (C) T_scm cells within T_n-like cells gated as shown in (A) or as (D) CD95^hi in HD and allo-SCT patients. (E) Memory subsets within antigen-specific T cells in healthy donor (CMV, n = 6) and allo-SCT patients (CMV, n = 5; MiHA, n = 3). ND, not detectable. Statistical analysis was performed by using (B,E) a 2-way analysis of variance (ANOVA) followed by a Bonferroni post hoc test or (C-D) a one-tailed Student t test. *P < .05; ***P < .001.