Domain structure and localization of PfPTP1 in P falciparum–infected RBCs. (A) Schematic of the PfPTP1 protein with its signal sequence in green, its PEXEL in red and the putative transmembrane domain in purple. Regions against which antibodies were raised are indicated with a black line. (B) PfPTP1 expression during the intracellular cycle (8-48 hours) in infected RBCs. (C) Localization of PfPTP1 during the intracellular cycle in immunofluorescence assay. The fixed cells were incubated with α-PTP1 antibody. Parasite nucleus was stained with DAPI. BF, bright field; ER, early ring; ET, early trophozoite; LR, late ring; LT, late trophozoite; MT, mid trophozoite, S, rupturing schizont.