Degree of ER colocalization of hVKORC1 variants. This bar graph represents the comparative mean Pearson’s coefficient and Mander’s R coefficient for wt and mutated hVKORC1 variants affecting the di-arginine and the di-lysine motifs analyzed in this study. Error bars represent the SD. The mean Pearson’s coefficient (gray bars) and Mander’s R (black bars) has been calculated from n = 10 to 24 ROIs. All calculations for Pearson’s and Mander’s R coefficient have been performed on the Image J version 4.3 visualization and analysis software. All hVKORC1 variants except Arg33Ala + Arg35Ala show a significant decrease in ER colocalization compared with the wt.