In vivo LV genomic integration profile in rapamycin-treated mouse bone marrow Lin⁻ cells. (A) Total number of unique LV integration sites (RIS) identified in BM Lin⁻ cells from primary transplant recipient mice described in Figure 2. *P < .05 from a parametric 2-tailed unpaired Student t test. (B) Percentage of the total number of RIS mapped to each chromosome. White, DMSO only; red, 5 μg/mL rapamycin. Asterisk (*) denotes borderline significant differences between the frequency of integration in chromosomes 7 and 13 (P = .077 and .076, respectively). (C) Genome distribution of identified integration sites. The mouse genome is represented by chromosomes 1 to 19, x and y clockwise from top. Inner circles represent all mice from a given treatment group: rapamycin-treated mice (first inner circle, warm colors) and control mice (second inner circle, cool colors). Each colored dot represents an individual integration event and is color-coded by the mouse in which it was identified within the specified treatment group. Integrations mapped to chromosomes 7 and 13 are magnified (center). For all panels, lines represent group mean and error bars represent standard deviation.