TF and thrombogenicity are upregulated in vivo in PARP-14–deficient mice, but TNFα expression is not affected. (A-E) WT and Parp14^−/− mice were studied unstimulated or 4 hours after intraperitoneal injection of LPS (5 μg) (n = 6-8 mice per group). (A) TNFα and (C) TF mRNA in lung tissue were measured by quantitative RT-PCR. (B) Serum TNFα was measured by enzyme-linked immunosorbent assay. (D-E) TF activity was measured in lung tissue and peripheral blood leukocytes using a turbimetric clotting assay (n = 6-8 mice per group). (F) Comparison by intravital microscopy of thrombus formation in cremaster arterioles. Each point represents average maximal thrombus volume normalized to vessel diameter for each individual mouse (n = 5 mice per group). All bars are medians. *P < .05; **P < .01. h, hours; NS, not significant using a 2-tailed Mann-Whitney U test.