Figure 2
Isolation of BFU-E and CFU-E cells by cell-sorting using CD34, CD36, and IL-3R as markers. (A) Colony forming ability of the sorted IL-3R+ and IL3R− cells. (B) Colony forming ability of the sorted IL-3R−CD34+CD36− in complete medium, EPO-only medium, and EPO+SCF+IL3 or EPO+SCF medium. (C) Colony forming ability of the sorted IL-3R−CD34−CD36+ cells in complete medium, EPO-only medium, and EPO+SCF+IL3 or EPO+SCF medium. (D) Expression of surface markers of sorted IL-3R−CD34+CD36− and IL-3R−CD34−CD36+ cells. The surface expression of indicated proteins was measured by flow cytometry. The representative profiles are shown. Gray line: autofluorescence control from unstained cells; black line: fluorescence from cells stained with indicated antibody. Results from 3 independent experiments are shown.

Isolation of BFU-E and CFU-E cells by cell-sorting using CD34, CD36, and IL-3R as markers. (A) Colony forming ability of the sorted IL-3R+ and IL3R cells. (B) Colony forming ability of the sorted IL-3RCD34+CD36 in complete medium, EPO-only medium, and EPO+SCF+IL3 or EPO+SCF medium. (C) Colony forming ability of the sorted IL-3RCD34CD36+ cells in complete medium, EPO-only medium, and EPO+SCF+IL3 or EPO+SCF medium. (D) Expression of surface markers of sorted IL-3RCD34+CD36 and IL-3RCD34CD36+ cells. The surface expression of indicated proteins was measured by flow cytometry. The representative profiles are shown. Gray line: autofluorescence control from unstained cells; black line: fluorescence from cells stained with indicated antibody. Results from 3 independent experiments are shown.

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