HIF-1α status does not affect the self-renewal of MLL-AF9 LICs but alters the extramedullary location of infiltrating leukemic cells. (A) Kaplan-Meier survival curve of secondary recipients transplanted with BM cells derived from leukemic mice of both genotypes (n = 6). The log-rank (Mantel-Cox) test was used to assess statistical significance. (B) Total weight of sex- and age-matched mice 3 weeks after transplantation (n = 4). The development of the disease was measured 3 weeks after transplantation in animals derived from 2 independent experiments by numbers of WBC (n = 16) (C) and the percentage of transduced cells (GFP⁺) (D) and myeloid cells (Gr1⁺ and/or Mac1⁺) (E) in PB (n = 16). A set of mice was sacrificed and stage of the disease analyzed by: spleen and liver weights (F), macroscopic examination of the livers (G), and GFP⁺ cells and myeloid cells (Gr1⁺ and/or Mac1⁺) in the BM and spleen (H) (n = 11). Plots represent mean ± SEM and boxes represent mean ± min-to-max values. Two-tailed Student t test was used to assess statistical significance. *P < .05, ***P < .001.