Hif-1α deletion accelerates early development of HOXA9-MEIS1–induced AML. (A) Kaplan-Meier survival curve of mice transplanted with HoxA9-Meis1–expressing cells (n = 10). The log-rank (Mantel-Cox) test was used to assess statistical significance. (B) Percentage of myeloid cells in the PB of transplanted animals at different time points after transplantation (week 3: n = 10; week 8: Hif-1α^Δ/Δ, n = 10; Hif-1α^fl/fl, n = 9; week 11: Hif-1α^Δ/Δ, n = 6; Hif-1α^fl/fl, n = 7). Two-way ANOVA was used to test statistical significance. (C) Representative FACS plots of PB cells at week 8 after transplantation showing an increment in the myeloid population of Hif-1α^Δ/Δ samples. The differentiated populations are stained with the following antibodies: CD3 for T cells (T), B220 for B cells (B), and Gr1/Mac1 for myeloid cells (M). Diseased mice were sacrificed at an advanced stage of disease and several parameters analyzed: percentage of myeloid cells in PB, BM, and spleen (D); WBC count (E); and spleen and liver weights (F) (Hif-1α^Δ/Δ, n = 9; Hif-1α^fl/fl, n = 6). (G) CFU assay derived from BM cells from both genotypes (n = 5). Plots and columns represent mean ± SEM. Unless otherwise stated, 2-tailed Student t test was used to assess statistical significance. *P < .05.