Fanca^−/− MKs present high level of nucleoplasmic bridges and DNA damage. (A) Representative view of multilobulated nuclei of WT and Fanca^−/− MKs after DAPI (left) and DAPI (red) plus Lamin A/C (green) staining (right; bar = 10 μm). Arrows indicate the location of NPBs. (B,C) Percentages of WT and Fanca^−/− MKs with NPBs (B, minimum of 80 cells scored per point, ***P < .001, Student t test) with the indicated number of NPBs per cell (C, n = 3, minimum of 100 cells scored per point, *P < .05, Student t test). (D) Representative images of MKs stained for 53BP1 (left) and percentages of 53BP1-positive MKs (right; n = 3, *P < .05, Student t test, ≥50 cells analyzed per point). Means ± SEM are shown; bar = 10 μm.