American Society of Hematology

Figure 2

$Figure 2. Proliferation and differentiation are altered in Fanca−/− MK progenitors. (A) Representative flow cytometry plots showing the gating strategy for MK progenitors. (B) Percentage of MK progenitors in Lin− cells of the BM of Fanca−/− and WT mice. (C) Proliferation curve (WT, n = 3; Fanca−/−, n = 4; results presented as means ± SEM, *P < .05, Student t test). (D) Number of MK-CFU colonies scored at day 6 of MegaCult-C from WT and Fanca−/− BM. (E) Number of MK-CFU scored at day 7 of MegaCult-C from LSKs transduced with an EGFP expressing vector (WT EGFP, Fanca−/− EGFP) or FANCA-EGFP vector (Fanca−/− FANCA) (n = 5 mice per group WT EGFP and Fanca−/− EGFP; n = 4 per group Fanca−/− FANCA, ***P < .001, *P < .05, Student t test). (F) Percentages of MKs obtained from nonfractionated nuclear WT and Fanca−/− BM cells after 3 days of culture with TPO (50 ng/mL, *P < .05, Student t test).$

Proliferation and differentiation are altered in Fanca^−/− MK progenitors. (A) Representative flow cytometry plots showing the gating strategy for MK progenitors. (B) Percentage of MK progenitors in Lin⁻ cells of the BM of Fanca^−/− and WT mice. (C) Proliferation curve (WT, n = 3; Fanca^−/−, n = 4; results presented as means ± SEM, *P < .05, Student t test). (D) Number of MK-CFU colonies scored at day 6 of MegaCult-C from WT and Fanca^−/− BM. (E) Number of MK-CFU scored at day 7 of MegaCult-C from LSKs transduced with an EGFP expressing vector (WT EGFP, Fanca^−/− EGFP) or FANCA-EGFP vector (Fanca^−/− FANCA) (n = 5 mice per group WT EGFP and Fanca^−/− EGFP; n = 4 per group Fanca^−/− FANCA, ***P < .001, *P < .05, Student t test). (F) Percentages of MKs obtained from nonfractionated nuclear WT and Fanca^−/− BM cells after 3 days of culture with TPO (50 ng/mL, *P < .05, Student t test).

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