Aberrant hypermaturation of neutrophils in zebrafish adult kidney hematopoiesis after deletion of miR-142-3p. (A) Flow cytometry analysis of WKM in wild-type (left panel) and 142ab^−/− (right panel) zebrafish. Myelomonocytes (mye) were indicated in red circle; hematopoietic progenitors (pro), blue circle; and lymphocytes and lymphocyte-like cells (lym), green circle. Average percentage ± SD for each fraction from 8 individual zebrafish at 65 dpf was shown. (B) Comparison of side scatter analysis (SSC) of wild-type and 142ab^−/− WKMs. Data were from 3 individual zebrafish in each group. (C) Wright-Giemsa staining of lyz-GFP⁺ myelomonocytes in wild-type (upper panel) or 142ab^−/− (lower panel) zebrafish kidney. Hypermature neutrophils were indicated with red triangle. Macrophage-like cells were indicated with black triangle. Bar represents 8 μm. (D-E) Quantification of cell size (D) and nucleocytoplasmic ratio (E) of neutrophils from wild-type and 142ab^−/− zebrafish kidney. (F-H) Quantification of the 3 hematopoietic fractions in wild-type and 142ab^−/− kidney with age. Hematopoietic progenitors (F), myelomonocytes (G), and lymphocytes and lymphocyte-like cells (H). WKM from at least 4 individuals was analyzed by flow cytometry in each group.*P < .05 or **P < .01 vs wild-type; 2-tailed Student t test.