The killing of unopsonized and opsonized C albicans by neutrophils. (A) Neutrophils from healthy controls were cocultured overnight with unopsonized and serum-opsonized C albicans. White arrows indicate the observed clusters of GFP-positive C albicans hyphae. Scale bar is 50 μm. (B) Neutrophils from healthy controls were cocultured overnight with unopsonized, serum-opsonized, HI-serum-opsonized, XLA-serum-opsonized, IgG-opsonized C albicans–GFP or after blocking the FcγRs with mAbs (10 μg/mL) and the clusters of hyphae were assessed microscopically. Results are means ± SEM of at least 3 independent experiments. *P < .05. HI, heat inactivated.