Difference in sensitivity to TAK1 inhibition between NBM CD34⁺ cells and AML CD34⁺ cells. (A) NBM CD34⁺ cells (n = 5) were incubated with 100 nM AZ-TAK1 for 24 hours and after 2 weeks CFCs were determined. (B) Cord blood CD34⁺ cells were transduced with hairpins targeting TAK1; after 2 weeks, CFCs were determined. (C) AML CD34⁺ cells (n = 8) and NBM CD34⁺ cells (n = 6) were incubated with 100 nM AZ-TAK1; after 24 hours cell death was quantified by annexin V staining.