IRAK-4 and MyD88 deficiencies impair T-independent IgM responses. Quantity of total and bacterial array carbohydrates bound by serum (A) IgM and (B) IgG. Control (n = 8), IRAK-4 deficient (n = 8), MyD88 deficient (n = 3). (C) Example CFG array results with dotted line illustrating RFU cutoff for bound carbohydrates. Bar represents mean; whiskers represent standard error of the mean. ***P < .001.