The immunoglobulin gene features of the 29 anti-ADAMTS13 mAbs. (A) Schematic organization of the immunoglobulin V(D)J junction: framework regions FR1 to 4 and CDR1 to 3, with CDR3 being the main antigen-binding site. Sites of random nontemplated nucleotide insertions locus at the V-D and D-J junctions are indicated in black. (B) Pie charts of the composition of the H-chain gene use for the IgV_HD_HJ_H segments and the percentage for the CDR3 amino acid length and amount of negatively charged amino acids. (C) Pie charts of the composition of the L-chain gene use for the IgV_LJ_L segments and the percentage for the L-chain CDR3 amino acid length and the amount of negatively charged amino acids.