Alignment of the deduced amino acid sequence of all 29 mAbs to their most homologous germ-line genes. Amino acid sequence of (A) the variable H-chain segments and (B) L-chain segments of phage display-derived IgG Fabs (panning round 5) and whole IgG of EBV-transformed switched memory B cells directed against ADAMTS13 generated from the spleens of 2 patients with relapsing acquired TTP. Clone identity: phage display-derived Fabs are indicated by italic letters and numbered with either 1 or 3 when originating from the κ library and 2 or 4 when originating from the λ library. Whole IgGs derived from EBV-transformed switched memory B cells are labeled with capital letters. According to the H-chain CDR3 amino acid sequences, all 29 mAbs are grouped into 8 CDR3 motifs; clones belonging to the same CDR3 motif are colored identically. The differences within 1 CDR3 motif are highlighted in red, dashes indicate missing, and letters indicate the replaced amino acids compared with the first top clone in each motif.