Validation of GEP data. (A-H) Indicated genes were validated with Q-RT-PCR and immunoblot. cHL cell lines expressing FOXO1(A3)ER (F1ER) or EV were treated with 4-OHT for 24 hours. Q-RT-PCR data were analyzed by the comparative Ct method. RPL13A was used as reference. mRNA expression levels are shown as fold change relative to untreated cells. A 2-sided Student t test was used to estimate statistical significance between treated and untreated samples. For TNFRSF8, MYC, and PRDM1, P values were <.05 in all cell lines. For BCL6, P values were <.05 except for SUP-HD1 (P = .06). For immunoblot, ACTB was used as loading control.